Salivary and serum inflammatory mediators among pre-conception women with periodontal disease

Background

There have been inconsistent conclusions regarding the levels of inflammatory mediators in saliva and serum among people with or without periodontal disease. Although pre-conception has been put forward as the optimal time for the periodontal treatment in order to improving pregnancy outcomes, few studies have been conducted to examine inflammatory mediators in saliva and serum among pre-conception women.

Methods

Pre-conception women were recruited between January 2012 and December 2014. Women were provided with an oral health examination to detect periodontal disease. Salivary and serum samples were collected at the same of examination. Inflammatory mediators includinginterleukin-1 beta (IL-1β), IL-6, tumor necrosis factor alpha (TNF-α) and beta-glucuronidase (β–glucuronidase) were tested and analyzed among women with overall periodontal disease (n?=?442) or moderate/severe periodontal disease (n?=?247). Results were compared to that in women with a healthy periodontium (n?=?91).

Results

Significantly increased concentrations of inflammatory mediators of IL-1β, IL-6, TNF-α and β-glucuronidase in saliva and IL-1β, β-glucuronidase and TNF-α in serum were found among pre-conception women with moderate/severe periodontal disease, compared with women without periodontal disease. Significantly increased levels were also found in all the above saliva inflammatory mediators and in serum IL-1β and TNF-α among women with overall periodontal disease. The levels of all inflammatory mediators in saliva and almost all inflammatory mediators except IL-6 in serum significantly increased with severity of periodontal disease.

Conclusion

Periodontal disease is highly associated with the elevated levels of inflammatory mediators in saliva and some mediators in serum among pre-conception women.

     

急性日本血吸虫病与血清白介素?33的关系研究

目的 目的 检测急性日本血吸虫病患者外周血血清白介素?33 （IL?33） 水平, 探讨IL?33在急性日本血吸虫病疾病进展中的作用。方法 方法 选择来自湖区的4例急性日本血吸虫病患者和来自非疫区的15例对照者作为研究对象, 收集人口学资料并采集肘静脉血。采用酶联免疫吸附试验检测所有研究对象的外周血血清IL?33水平。应用Stata 10.0软件进行数据的统计学分析。 结果 结果 急性日本血吸虫病患者血清IL?33水平为517.33 （334.65,1 056.88）pg/ml, 显著高于对照者血清IL?33 水平1.66 （1.66,6.35）pg/ml （Z= -3.207,P = 0.001）。急性日本血吸虫病患者血清IL?33水平与外周血嗜酸粒细胞计数、 患者感染时长的相关系数均为0.8, 相关系数均无统计学意义 （P = 0.2）。结论 结论 IL?33在急性日本血吸虫病患者血清中显著升高, 在日本血吸虫病急性期发挥了促进炎症作用, 并可能在患者感染日本血吸虫后7~9周参与启动Th2型免疫应答。     

HIV与血吸虫共同感染的研究进展

关艾滋病与血吸虫病的研究已较深入，但关于两种病原体共同感染的报道较少。早在1990年就有学者提出血吸虫感染动物产生的抗体能特异地与HIV的一种蛋白质     

上海市医用回旋加速器共享现况分析

目的:了解上海市目前医用回旋加速器的共享现况及存在的问题。方法:选取上海市配备回旋加速器的4所公立医院和未配备回旋加速器的3所公立医院为样本,开展机构调查和访谈。结果:上海市医疗机构共享回旋加速器的模式有四种:主动共享、被动共享、自用+被动共享和非共享型。共享中存在的主要问题为三甲医院缺乏建立共享机制的动力、缺乏进一步拓展共享的空间、尚没有形成统一、规范的资源共享网络以及医疗机构间的资源共享存在着一定的利益冲突。结论:上海市公立医院之间尚未建立起良好的回旋加速器共享机制,建议在现有回旋加速器共享网络的基础上逐步建立共享平台,促进资源的合理配置和使用。     

STRmix™ collaborative exercise on DNA mixture interpretation

An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded.For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 × 10⁴ to 8 × 10⁶. For Sample 2 (in the order of 2000 rfu for major contributors), LRs ranged from 2 × 10²⁸ to 2 × 10²⁹. Where LRs were calculated, the differences between participants can be attributed to (from largest to smallest impact):

• •varying number of contributors (NoC),
• •the exclusion of some loci within the interpretation,
• •differences in local CE data analysis methods leading to variation in the peaks present and their heights in the input files used,
• •and run-to-run variation due to the random sampling inherent to all MCMC-based methods.

This study demonstrates a high level of repeatability and reproducibility among the participants. For those results that differed from the mode, the differences in LR were almost always minor or conservative.     

MPO、NQO1、GSTP1和UGT1A6基因多态与慢性苯中毒遗传易感性关系

目的探讨MPO、NQO1、GSTP1和UGT1A6基因多态与慢性苯中毒易感性的关系。方法采用病例-对照研究,以268名苯中毒工人为病例组,268名接触苯而没有中毒表现的工人为对照组。应用TaqManPCR分析技术判定MPO(rs7208693),NQO1(rs1800566),GSTP1(rs947894)和UGT1A6(rs6759892,rs1105879,rs4124874,rs3755319,rs887829和rs4148323)基因型。结果携带GSTP1基因rs947894G等位基因个体患慢性苯中毒的危险性比AA基因型个体降低0.657倍(95%CI0.434~0.994,P=0.046);携带MPO基因rs7208693A等位基因人群中,UGT1 A6 rs6759892G等位基因个体发生慢性苯中毒的危险性是TT基因型的2.702倍(P=0.024),UGT1 A6 rs1105879C等位基因个体发生慢性苯中毒的危险性是TT型的2.619倍(P=0.035)。在饮酒人群中,携带NQO1基因rs1800566TT基因型个体慢性苯中毒的发病风险较携带CC和CT基因型个体增加9.000倍(95%CI1.460~55.478,P=0.021);在吸烟人群中,带NQO1基因rs1800566TT基因型个体慢性苯中毒的发病风险较携带CC和CT基因型个体增加7.000倍(95%CI1.555~31.575,P=0.012)。单倍型分析显示,本人群携带UGT1A6基因TACGGG单倍型个体慢性苯中毒的发病风险是携带TAATGG单倍型个体的1.446倍(OR=1.446,95%CI1.005~2.080,P=0.046)。结论同时携带MPO基因rs7208693A和UGT1A6基因rs6759892G或rs1105879C等位基因型个体对苯中毒易感;携带NQO1基因rs1800566TT基因型且同时吸烟或饮酒的个体对苯中毒易感;携带UGT1A6基因TAATGG单倍型个体可增加慢性苯中毒的发病风险;GSTP1基因多态与慢性苯中毒遗传易感性的关系仍需进一步研究。     

S型氯代甘油醇对雄性ICR小鼠生育力的影响

目的研究S型氯代甘油醇对雄性ICR小鼠生育能力及睾丸、附睾的组织病理学影响。方法雄性性成熟ICR小鼠64只,随机分为4组,每组16只。实验组分别给予1/40、1/20、1/10LD50(小鼠经口LD50117.54mg/kg)S型氯代甘油醇,对照组给予等体积双蒸水。连续灌胃染毒5天后,每组取4只雄鼠按雌/雄1∶1交配,此后每隔2天交配一次,持续2个月,观察S型氯代甘油醇对雄性小鼠交配指数和雄性生育力指数的影响。实验结束后,每组保留6只动物继续染毒至8个月后进行附睾精子计数和睾丸、附睾的组织病理学检查。结果①与对照组相比,染毒S型氯代甘油醇2个月未引起雄性ICR小鼠交配指数和雄性生育力指数显著降低(P>0.05);②染毒8个月未导致附睾精子数量显著降低(P>0.05),睾丸和附睾的组织病理学检查未见明显异常。结论低剂量长期染毒S型氯代甘油醇对雄性小鼠不具有抗生育作用。     

凉山彝族聚居区育龄妇女HIV/AIDS感染横断面研究

凉山彝族自治州(凉山州)是我国目前艾滋病疫情最严重的地区之一^[1]。近年来,随着艾滋病性传播比例上升,女性HIV感染者数量不断增多。由于凉山州独特的地理人文环境仍有很多女性尚未认识到HIV感染的危害或因感染HIV未及时被发现,成为新的传染源,导致配偶胜伴以及后代的感染。为了解凉山州彝族聚居区育龄妇女HIV感染水平及其相关危险因素,本研究开展HIV/AIDS横断面调查。